Meric, GoekceGuvenir, MeryemSuer, Kaya2024-05-252024-05-2520170001-63571502-385010.1080/00016357.2017.13313742-s2.0-85019721225https://doi.org/10.1080/00016357.2017.1331374https://hdl.handle.net/20.500.14517/514Süer, Kaya/0000-0002-2565-3425; guvenir, meryem/0000-0002-9702-9947Objective: To compare the effectiveness of commonly used herbal/non-fluoride with fluoride dentifrices in order to eliminate pathogenic oral microorganisms from denture base material. Materials and methods: Heat-polymerized acrylic resin specimens (n = 288) were divided into three groups and each group inoculated with three various microorganisms (n = 96 for each) Candida albicans, Staphylococcus aureus and Pseudomonas aeruginosa (P. aeruginosa). Contaminated specimens were randomly assigned to the application of six herbal/non-fluoride and three fluoride dentifrices. These specimens were divided into two groups: negative and positive control (n = 3 for each). All acrylic specimens were incubated at 37 degrees C for 24 h for samples inoculated with bacterial strains and 37 degrees C for 48 h for samples inoculated with yeast strains. After the incubation period, all brain-heart infusion broths that contained disinfectant acrylic specimens were cultured on 5% sheep blood agar for bacterial counts and Sabouraud dextrose agar for yeast counts. The number of colony-forming units per millilitre (CFU/mL) were calculated. The results were analysed by Mann-Whitney U and Kruskal-Wallis tests (p = .05). Results: Both herbal/non-fluoride and fluoride dentifrices were effective against Candida albicans. However, fluoride dentifrices were comparatively better than the herbal/non-fluoride dentifrices against Staphylococcus aureus and P. aeruginosa. Conclusions: Herbal dentifrices could be used, especially among the elderly who lack a degree of manual dexterity during the rinsing of dentifrice chemicals from their dentures.eninfo:eu-repo/semantics/closedAccessAcrylic resinmicroorganismgerodontologyArticle0