Kurt, Enes A.Demirci, MehmetOzbey, DogukanGareayaghi, NesrinEskazan, TugceErzin, Yusuf Z.Kocazeybek, BekirTıbbi Mikrobiyoloji / Medical MicrobiologyTıbbi Mikrobiyoloji / Medical Microbiology2024-05-252024-05-25202401433-651010.7754/Clin.Lab.2023.2307112-s2.0-85182098936https://doi.org/10.7754/Clin.Lab.2023.230711https://hdl.handle.net/20.500.14517/1120kurt, enes ali/0000-0001-8056-4428Background: Crohn's disease (CD) and ulcerative colitis (UC) are inflammatory bowel diseases with uncertain etiology. We aimed to determine the amounts of Akkermansia muciniphila and Faecalibacterium prausnitzii in the intestinal microbiota of these patients and to correlate their amounts with blood IL-8, IL-10, and IL-12 cytokine levels. Methods: Thirty UC, 30 CDs, and 46 healthy controls were included. IL-8, IL-10, and IL-12 levels of blood samples were analyzed by ELISA. The amounts of Akkermansia muciniphila and Faecalibacterium prausnitzii were determined by the LightCycler 480 qPCR system. Results: F. prausnitzii, A. muciniphila, IL-10, and IL-12 decreased in patient groups, while IL-8 decreased in UC but increased in CD. A significant difference was detected between the patient and control groups in terms of F. prausnitzii, A. muciniphila, and IL-8, but not for others. The amount of F. prausnitzii was correlated with IL-8 and IL-10 in UC and with IL-10 in CD patients. Conclusions: The decrease in the amount of F. prausnitzii was associated with the increase in UC disease severity. A. muciniphila and F. prausnitzii were detected in lower amounts in both diseases. F. prausnitzii decreased more with the severity of UC, suggesting that these bacteria may have complex roles in their etiopathogenesis.eninfo:eu-repo/semantics/closedAccessUlcerative ColitisCrohn's Diseasegut microbiotaAkkermansia muciniphilaFaecalibacterium prausnitziiArticleQ4Q3701157166WOS:00117880790002038213219