Guler, SevkiCetinkaya, Burcu OzkanKurt Bayrakdar, SevdaAyas, BulentKeles, Gonca CayirPeriodontoloji / Periodontology2024-05-252024-05-25202201354-523X1601-082510.1111/odi.138522-s2.0-85103933341https://doi.org/10.1111/odi.13852https://hdl.handle.net/20.500.14517/2114KURT BAYRAKDAR, Sevda/0000-0002-3711-6520Objectives The present study was performed to compare the effectiveness of Ankaferd Blood Stopper(R) (ABS) with enamel matrix derivatives (EMD) for treating fenestration defects in rats. Materials and Methods Forty-eight male Wistar rats were randomly divided into six groups (each n = 8). Fenestration defects were created in all rats, to which ABS, EMD, or saline (S) was then applied. The rats were grouped and sacrificed at one of two different time points, as follows: ABS-10-group, ABS-treatment/sacrifice on day 10; EMD-10-group, EMD-treatment/sacrifice on day 10; S-10-group, S-treatment/sacrifice on day 10; ABS-38-group, ABS-treatment/sacrifice on day 38; EMD-38-group, EMD-treatment/sacrifice on day 38; and S-38-group, S-treatment/sacrifice on day 38. Then, histomorphometric analysis including measurements of new bone area (NBA) and new bone ratio (NBR), and immunohistochemical analysis including the determination of osteopontin (OPN) and type-III-collagen (C-III) expression were performed. Results The NBA and NBR were significantly higher in the ABS-10-group and EMD-10-group compared to the S-10-group (p < .05), and in the EMD-38-group compared to the S-38-group (p < .05). The levels of C-III and OPN immunoreactivity were significantly higher in the ABS-10-group compared to the S-10-group (p < .017). Conclusions The results of this study suggested that ABS can promote early periodontal regeneration, although its efficacy seems to decrease over time.eninfo:eu-repo/semantics/closedAccessAnkaferd Blood StopperEmdogainhistomorphometricosteopontintype&#8208III collagenArticleQ1Q128719471957WOS:00063554390000133740823