Yenier Yurdagüven, G.Tarim, B.2025-07-152025-07-1520232148-752910.15311/selcukdentj.12359992-s2.0-105007698390https://doi.org/10.15311/selcukdentj.1235999https://hdl.handle.net/20.500.14517/8098Background: The aim of this study was to evaluate the effect of different adhesive applications on dentine microtensile bond strength (μTBS) in simplified adhesive systems. Methods: Seventy-two extracted non-carious human third molars were randomly divided 18 groups (n=4). Dentin surfaces were polished with #600-grit silicon-carbide paper. Two-step etch&rinse Adper Single Bond2 [SB](3M ESPE) and one-step self-etch Clearfil S3 Bond [S3](Kuraray) were tested, two-step self-etch Clearfil SE Bond [SE](Kuraray) was used as control. The tested adhesives were applied according to the manufacturer’s instructions [MI] or with 2% Chlorhexidine [CHX] or double application of the adhesive [DA] or with hydrophobic resin layer [HR]. Composite build-up (Filtek Ultimate,3MESPE) was restored incrementally and stored in distilled water (24h/37°C). Teeth were sectioned as 1mm2 resin-dentin sticks (Isomet) and subjected to μTBS test (Bisco) immediately [24h] or after six months of water-storage [6M] (Crosshead speed:0.5mm/min.) Data were analyzed by Three-way ANOVA and post-hoc Tukey’s tests (p<0.05). Results: The dentine µTBS was significantly influenced by the adhesive system (p:0.001), adhesive applications (p:0.001), and storage time (p:0.001). The highest μTBS was found in SE-MI (p<0.05), while the interaction between SB-MI and S3-MI was not significant (p>0.05). The µTBS of all groups decreased significantly at 6M (p<0.01). HR and DA showed significantly higher μTBS than CHX and MI (p<0.01), while no significant difference was found between CHX and MI (p>0.05). When adhesives were compared, DA and HR were significantly higher μTBS in S3 (p<0.01). Conclusion: HR and DA improved the μTBS while no significant enhancement was observed by CHX. © 2023, Selcuk University. All rights reserved.trinfo:eu-repo/semantics/closedAccessChlorhexidineDental AdhesivesDentinDentin Bonding AgentsLongevityArticleN/AN/A104 Special Issue210217