Olcay, KezibanTasli, Pakize NeslihanGuven, Esra PamukcuUlker, Gul Merve YalcinOgut, Emine EsenCiftcioglu, ElifSahin, FikrettinEndodonti / EndodonticsAğız,Diş ve Çene Cerrahisi / Oral, Dental and Maxillofacial Surgery2024-05-252024-05-252020171678-77571678-776510.1590/1678-7757-2019-02152-s2.0-85077842779https://doi.org/10.1590/1678-7757-2019-0215https://hdl.handle.net/20.500.14517/1360Öğüt, Emine/0000-0002-8137-6952; Taşlı, Neslihan/0000-0002-0455-9894; Ciftcioglu, Elif/0000-0002-2578-0168; PAMUKCU, Prof. Dr. ESRA/0000-0003-2714-009X; Yalcin, Gul Merve/0000-0002-7438-5834; Sahin, Fikrettin/0000-0003-1503-5567; Olcay, Keziban/0000-0002-2168-710XObjective: This study evaluated the angiogenesis-enhancing potential of a tricalcium silicate-based mineral trioxide aggregate (ProRoot MTA), Biodentine, and a novel bioceramic root canal sealer (Well-Root ST) in human dental pulp stem cells (hDPSCs), human periodontal ligament stem cells (hPLSCs), and human tooth germ stem cells (hTGSCs). Methodology: Dulbecco's modified Eagle's medium was conditioned for 24 h by exposure to ProRoot MTA, Biodentine, or Well-Root ST specimens (prepared according to the manufacturers' instructions). The cells were cultured in these conditioned media and their viability was assessed with 3-(4,5-dimethyl-thiazol-2-yl)-5-(3-carboxy-methoxy-phenyl)-2-(4-sulfo-phenyl)-2H tetrazolium (MTS) on days 1, 3, 7, 10, and 14. Angiogenic growth factors [platelet-derived growth factor (PDGF), basic fibroblast growth factor (FGF-2), and vascular endothelial growth factor (VEGF)] were assayed by sandwich enzyme-linked immunosorbent assay (ELISA) on days 1, 7, and 14. Human umbilical vein endothelial cell (HUVEC) migration assays were used to evaluate the vascular effects of the tested materials at 6-8 h. Statistical analyses included Kruskal-Wallis, Mann-Whitney U, and Friedman and Wilcoxon signed rank tests. Results: None of tricalcium silicate-based materials were cytotoxic and all induced a similar release of angiogenic growth factors (PDGF, FGF-2, and VEGF) (p>0.05). The best cell viability was observed for hDPSCs (p<0.05) with all tricalcium silicate-based materials at day 14. Tube formation by HUVECs showed a significant increase with all tested materials (p<0.05). Conclusion: The tricalcium silicate-based materials showed potential for angiogenic stimulation of all stem cell types and significantly enhanced tube formation by HUVECs.eninfo:eu-repo/semantics/openAccessAngiogenesis inducing agentsDental cementsRegenerativeendodonticsStem cellsArticleQ2Q128WOS:00050968920000131939521