Browsing by Author "Altunkaynak,B.Z."

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Citation Count: 0
The effects of bortezomib on the ovariectomy applied rat uterus: A histopathological, stereological, and immunohistochemical study
(Mashhad University of Medical Sciences, 2018) Khoshvaghti,H.; Altunkaynak,B.Z.
Objective(s): In this study, potential protective effects of Bortezomib (Bort), as a proteasome inhibitor, were investigated on the uterus of ovariectomized rats by histological, morphometric and immunohistochemical methods. Materials and Methods: In this study, 18 Sprague dawley strain female rats (12 weeks old, 250-300 g body weight) were used. Animals in the control group (Cont, n=6) were not exposed to any treatment. Ovariectomy was performed on the experimental groups. They (n=12) were divided into ovariectomy (Ovt, n=6) and Bortezomib (Bort, n=6) subgroups. Twelve weeks later, the rats were perfused. Then, uterine tissues were removed and examined by morphometrical, and light and electron microscopy methods. In addition, immunoreactivity of nuclear factor-kappa (NF-κB) was evaluated. Results: Morphometric and histopathological evaluations showed that Bort was effective in the uterus and protects the layer structures and the cells. Conclusion: In the light of these findings, we suggest that for proteasome inhibitor particularly Bort is thought to be useful through proteasome inhibition and NF-κB pathway. © 2018, Mashhad University of Medical Sciences. All rights reserved.
Citation Count: 1
Evaluation of the protective effects of folic acid on the lung exposed to 900-MHZ electromagnetic field: A stereological and histopathological study
(Ondokuz Mayis Universitesi, 2022) Tüfekçi,K.K.; Bakirhan,E.G.; Altunkaynak,B.Z.
There is strong scientific evidence that radio frequency (RF) radiation is harmful to life. Exposure to radiation may cause lung toxicity and respiratory disorders. Folic acid (FA) is one of the powerful antioxidants that minimize oxidative stress in the biological system. In this study, we evaluated the effectiveness of the FA against the EMF-induced potential negative effects on the lung. Twenty-four male Wistar albino rats were divided into the four groups; control group (Cont), electromagnetic field group (EMF), FA treated group (FA), and electromagnetic field exposure + FA treated group (EFA). After the routine histological procedures, volumes of the alveoli, bronchioles and blood vessels have been estimated by the Cavalieri principle. It was found that a significant decrease in the mean volume of alveoli, bronchioles and blood vessels in EMF group in comparison of the Cont group (p<0.01). Besides this, histopathological analysis demonstrated that there was impaired lung structure, shrunken alveoli, and increased thickness of the alveolar wall in the EMF group sections. In the EFA group, significant protective effects were observed in the structures volumes and histopathology (p<0.01). These findings corresponded with the antioxidant effect of FA treatment. Our results suggest that FA protected alveoli, bronchioles, and blood vessels against EMF-induced lung injury. Thus FA has the potential to be a therapeutic agent. © 2022 Ondokuz Mayis Universitesi. All rights reserved.
Citation Count: 0
Momordica charantia Enhances Tendon Healing in Rats: An Experimental Study
(S. Karger AG, 2024) Erdoğan,F.; Kaplan,A.A.; Coşkun,H.S.; Altun,G.; Altunkaynak,B.Z.; Kelsaka,E.; Pişkin,A.
Momordica charantia (MC) is a traditional plant widely used since ancient times for wound healing. This study evaluated its potential effects on tendon healing. Adult male Wistar albino rats (n = 32, 8 rats in each group) were anesthetized, and their Achilles tendons were prepared for surgical procedures. Group 1 (Cont = control group) was not subjected to any surgery and was used as a control group for baseline values. Group 2 (PR = primary repair group) underwent primary repair (PR) with a monofilament suture after a full-thickness incision of the Achilles tendon. A full-thickness incision was also made to the Achilles tendon of group 3 (CT = collagen tube-administered group), followed by PR and collagen tube insertion. In group 4 (MC = M. charantia-administered group), 1 mL of MC extract was applied locally on the collagen tube in addition to the surgical procedure applied to group 3. The Achilles tendons were excised on the postoperative 40th day and examined stereologically, histologically, and bioinformatically. Data showed that the total volume of the collagen fibers was higher in MC and CT groups than in the PR group. The total volume of the tendon was decreased in MC and CT groups than in the Cont group. The ratios between the volumes of the collagen fibers and total tendon in the MC and CT groups were significantly different from PR, but not different from the Cont group. Additionally, MC improved tenoblastic activity, collagen production, and neovascularization. Bioinformatic interactions showed that the proteases of MC could trigger the signals playing a role on vasculogenesis, reducing inflammation, and contributing to tenoblast activation and collagen remodeling. MC extract ameliorates the healing of injured tendon and can provide satisfactory tendon repair. Further works are recommended to explore the healing capacity of MC. © 2023 S. Karger AG, Basel.
Citation Count: 4
Protective effect of melatonin on the rat lung following exposure to 900-MHz electromagnetic field: a stereological and histopathological study
(Ondokuz Mayis Universitesi, 2021) Yahyazadeh,A.; Kivrak,E.G.; Koç,G.E.; Altunkaynak,B.Z.
Long-term use of cell phone emitting electromagnetic field (EMF) has increased concerns regarding histopathological change in vital organs. Our present study was aimed to investigate the effect of 900 megahertz (MHz) EMF on the rat lungs, as well as the possible efficacy of melatonin (MEL) on lung tissues. Fifteen female Wistar albino rats were randomly selected and assigned into five groups as follow: control (CONT), SHAM, EMF, melatonin (MEL) and EMF+MEL group. Subsequently, female rats were then mated, and pregnant rats underwent the experimental application for 21 days. After parturition, 6 pups (2 pups from each mother) were randomly chosen and maintained for 4 weeks. At the end of the 4th week, the lung tissues of all groups were used for the stereological analysis and histopathological examination. We found that the mean volumes of the alveoli, bronchioles and blood vessels were significantly lower in the EMF group compared to the control group (p<0.05). In the EMF+MEL group, the mean volumes of the alveoli, bronchioles and blood vessels were significantly increased compared to the EMF group. Our histopathological results showed marked change in the lung tissues. We speculated that exposure to EMF caused the damage to the rat lung tissues, and that MEL administration alleviated 900 MHz EMF-induced complications. © 2021 Ondokuz Mayis Universitesi. All rights reserved.
Citation Count: 4
Stereological and histological assessment of the umbilical cord in new-born rat
(Wolters Kluwer Medknow Publications, 2021) Altunkaynak,B.Z.; Yahyazadeh,A.
Background: Umbilical cord plays a crucial role in the continuation of pregnancy by transferring nutrition and oxygen across the placenta to the fetus. We aimed to investigate the morphometrical and histological features of the umbilical cords in new-born rats. Materials and Methods: The adult male and female rats were chosen for matting purpose in the present study. Briefly, ten adult Wistar albino rats (female, n = 5; male, n = 5) were randomly assigned into five groups of two animals (female, n = 1; male, n = 1). Immediately after parturition, two umbilical cords of new-born rats (0-day-old) from each group were randomly selected. Finally, ten umbilical cord samples were examined using the histological and stereological methods in the light and electron microscopes. Results: The total numbers of Hofbauer cells and mesenchymal stromal cells was estimated statistically. We also calculated the mean volume of umbilical cords, arteries and veins, as well as arterial and venous lumens. Our histological findings also exhibited the histological features of Hofbauer cells, mesenchymal stromal cell cells, and blood vessels. Conclusion: Our findings showed more detailed information about umbilical cord tissues and their components, and that may contribute to the diagnose of umbilical cord complications in the developing fetus. © 2020 Journal of Microscopy and Ultrastructure