Browsing by Author "Kaplan, Suleyman"

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Citation Count: 14
Biochemical, immunohistochemical and morphometrical investigation of the effect of thymoquinone on the rat testis following exposure to a 900-MHz electromagnetic field
(Elsevier Gmbh, 2020) Altunkaynak, Berrin Zühal; Altunkaynak, Berrin Zuhal; Kaplan, Suleyman; Histoloji ve Embriyoloji / Histology and Embriology
Long-term use of cell phones emitting electromagnetic fields (EMFs) have raised concerns regarding public health in recent year. We aimed to investigate the possible effects of 900 MHz EMF exposure (60 min/day for 28 days) on the rat testis. Another objective was to determine whether the deleterious effect of EMF radiation would be reduced by the administration of thymoquinone (TQ) (10 mg/kg/day). Twenty-four male adult Wistar albino rats were randomly selected, then assigned into four groups as followControl, EMF, TQ and EMF + TQ. Testicular samples were analyzed using histological, stereological, biochemical and immunohistochemical techniques. Total numbers of primary spermatocytes and spermatids as well as Leydig cells were significantly decreased in the EMF group compared to the Control group (p < 0.05). In the EMF + TQ group, the total number of primary spermatocytes was significantly increased compared to the EMF group (p < 0.05). Superoxide dismutase (SOD) activity was significantly increased in the EMF group compared to the Control group (p< 0.05). Also, serum testosterone levels and wet weight of testes were significantly decreased in the EMF group compared to the Control group (p < 0.05). Our findings suggested that exposure to a 900 MHz EMF had adverse effects on rat testicular tissue and that the administration of TQ partially mitigated testicular oxidative damages caused by EMF radiation.
Citation Count: 1
The effects of different doses of diclofenac sodium on newborn rat hippocampus exposed during the third trimester
(Tubitak Scientific & Technological Research Council Turkey, 2020) Altunkaynak, Berrin Zühal; Kaplan, Suleyman; Altun, Gamze; Aksoy, Abdurrahman; Altunkaynak, Berrin Zuhal; Histoloji ve Embriyoloji / Histology and Embriology
The main aim of the present study was to examine the neurotoxic effects of prenatal exposure to varying doses of diclofenac sodium (DS) on the rat hippocampus. Twenty-eight Wistar albino adult female rats weighing 280-300 g were initially used for pregnancy. When vaginal plaques were seen in female rats, it was accepted as the 0th day of pregnancy. Female rats were divided into five main groups; pure control, saline, low dose DS (3.6 mg/kg), moderate dose DS (9 mg/kg), and high dose DS (18 mg/kg). They were exposed to these treatments during their gestation. After birth, all newborn male rats were euthanized by overdose anesthesia on the 7th postnatal day. Histological and stereological techniques were used for analyzing tissue samples. The stereological analyses in this study showed that the number of neurons in the hippocampus may be reduced due to the use of DS. Histological investigation of the drug-treated groups showed significant cell loss compared to the control. Darkly stained nuclei of the neurons in the high dose group compared to the other groups were also obvious. When the results are evaluated, it can be concluded that the use of diclofenac sodium during pregnancy may have teratogenic effects on the development of the nervous system.
Citation Count: 7
Is melatonin, leptin or their combination more effective on oxidative stress and folliculogenesis in the obese rats?
(Taylor & Francis inc, 2020) Altunkaynak, Berrin Zühal; Altunkaynak, Berrin Zuhal; Kaplan, Suleyman; Histoloji ve Embriyoloji / Histology and Embriology
In this study, we evaluated the effects of melatonin (Mel), leptin (Lep) or melatonin and leptin treatment on ovaries in control and obese rats. The animals were divided into control (NC), melatonin (NM), leptin (NL), melatonin?leptin (NML), obese (OC), obese?melatonin (OM), obese?leptin (OL), obese?melatonin?leptin (OML) groups. Body weights, peri-ovarian fat pads, volumetric parameters and numerical values of follicles were estimated. Also, the LH receptor (LHr) immune-positivity, catalase (CAT) and the myeloperoxidase (MPO) levels were determined. The body weight and peri-ovarian fat pads were significantly decreased following Mel (p?