Assessment of Stiffness-Dependent Autophagosome Formation and Apoptosis in Embryonal Rhabdomyosarcoma Tumor Cells

dc.authorscopusid57305831500
dc.authorscopusid58528565900
dc.authorscopusid56700291100
dc.authorscopusid57202500098
dc.authorscopusid7404625308
dc.authorscopusid9732937200
dc.authorscopusid9732937200
dc.contributor.authorSezen, S.
dc.contributor.authorAdiguzel, S.
dc.contributor.authorZarepour, A.
dc.contributor.authorKhosravi, A.
dc.contributor.authorGordon, J.W.
dc.contributor.authorGhavami, S.
dc.contributor.authorZarrabi, A.
dc.date.accessioned2025-03-15T20:27:36Z
dc.date.available2025-03-15T20:27:36Z
dc.date.issued2025
dc.departmentOkan Universityen_US
dc.department-tempSezen S., Department of Molecular Biology, Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, Sabanci University, Tuzla, Istanbul, Turkey, Nanotechnology Research and Application Center, Sabanci University, Tuzla, Istanbul, Turkey; Adiguzel S., Nanotechnology Research and Application Center, Sabanci University, Tuzla, Istanbul, Turkey; Zarepour A., Department of Research Analytics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India; Khosravi A., Department of Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, Istanbul Okan University, Istanbul, Turkey; Gordon J.W., Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada; Ghavami S., Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada, Faculty of Medicine in Zabrze, University of Technology in Katowice, Zabrze, Poland, Research Institute of Oncology and Hematology, Cancer Care Manitoba, University of Manitoba, Winnipeg, MB, Canada; Zarrabi A., Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Istinye University, Istanbul, Turkey, Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Taoyuan, Taiwanen_US
dc.description.abstractRemodeling of the extracellular matrix (ECM) eventually causes the stiffening of tumors and changes to the microenvironment. The stiffening alters the biological processes in cancer cells due to altered signaling through cell surface receptors. Autophagy, a key catabolic process in normal and cancer cells, is thought to be involved in mechano-transduction and the level of autophagy is probably stiffness-dependent. Here, we provide a methodology to study the effect of matrix stiffness on autophagy in embryonal rhabdomyosarcoma cells. To mimic stiffness, we seeded cells on GelMA hydrogel matrices with defined stiffness and evaluated autophagy-related endpoints. We also evaluated autophagy-dependent pathways, apoptosis, and cell viability. Specifically, we utilized immunocytochemistry and confocal microscopy to track autophagosome formation through LC3 lipidation. This approach suggests that the use of GelMA hydrogels with defined stiffness represents a novel method to evaluate the role of autophagy in embryonal rhabdomyosarcoma and other cancer cells. © 2024. Springer Science+Business Media, LLC.en_US
dc.identifier.citation0
dc.identifier.doi10.1007/7651_2024_538
dc.identifier.endpage287en_US
dc.identifier.issn1940-6029
dc.identifier.pmid38647864
dc.identifier.scopus2-s2.0-85219501168
dc.identifier.scopusqualityQ4
dc.identifier.startpage275en_US
dc.identifier.urihttps://doi.org/10.1007/7651_2024_538
dc.identifier.urihttps://hdl.handle.net/20.500.14517/7744
dc.identifier.volume2879en_US
dc.identifier.wosqualityN/A
dc.language.isoenen_US
dc.relation.ispartofMethods in molecular biology (Clifton, N.J.)en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAutophagyen_US
dc.subjectEmbryonal Rhabdomyosarcomaen_US
dc.subjectGelma Hydrogelen_US
dc.subjectImmunocytochemistryen_US
dc.subjectStiffnessen_US
dc.subjectTumor Microenvironmenten_US
dc.titleAssessment of Stiffness-Dependent Autophagosome Formation and Apoptosis in Embryonal Rhabdomyosarcoma Tumor Cellsen_US
dc.typeArticleen_US
dspace.entity.typePublication

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