Determining the expression levels of circulating tumour cell markers in canine mammary tumours

dc.authoridDegirmencioglu, Sevgin/0000-0001-7243-3671
dc.authoridInal Gultekin, Guldal/0000-0002-8313-6119
dc.authoridUçmak, Zeynep Günay/0000-0003-2530-1291
dc.authorscopusid57217061843
dc.authorscopusid36632450400
dc.authorscopusid54580884800
dc.authorscopusid25632114400
dc.authorscopusid6603287153
dc.authorscopusid58570213500
dc.authorwosidGUVENC, KAZIM/GQZ-4338-2022
dc.authorwosidDegirmencioglu, Sevgin/ABI-3962-2020
dc.authorwosiddegirmencioglu, sevgin/M-4010-2017
dc.authorwosidInal Gultekin, Guldal/AAF-5392-2021
dc.authorwosidUçmak, Zeynep Günay/AAG-7917-2020
dc.contributor.authorİnal Gültekin, Güldal
dc.contributor.authorKahraman, Ozlem Timirci
dc.contributor.authorGultekin, Guldal Inal
dc.contributor.authorDegirmencioglu, Sevgin
dc.contributor.authorYaylim, Ilhan
dc.contributor.authorGuvenc, Kazim
dc.contributor.otherFizyoloji / Physiology
dc.date.accessioned2024-05-25T11:42:15Z
dc.date.available2024-05-25T11:42:15Z
dc.date.issued2021
dc.departmentOkan Universityen_US
dc.department-temp[Ucmak, Zeynep Gunay; Guvenc, Kazim] Istanbul Univ Cerrahpasa, Dept Obstet & Gynaecol, Fac Vet Med, Istanbul, Turkey; [Kahraman, Ozlem Timirci; Yaylim, Ilhan] Istanbul Univ, Aziz Sancar Inst Expt Med, Dept Mol Med, Istanbul, Turkey; [Gultekin, Guldal Inal] Istanbul Okan Univ, Dept Physiol, Fac Med, Istanbul, Turkey; [Degirmencioglu, Sevgin] Istanbul Arel Univ, Dept Biochem, Fac Med, Istanbul, Turkeyen_US
dc.descriptionDegirmencioglu, Sevgin/0000-0001-7243-3671; Inal Gultekin, Guldal/0000-0002-8313-6119; Uçmak, Zeynep Günay/0000-0003-2530-1291en_US
dc.description.abstractDetection of the circulating tumour cells (CTC) in dogs with a mammary tumour is a useful tool to reveal the micrometastases long before metastases are recognised clinically. The aim of this study was to evaluate the association of the epidermal growth factor receptor (EGFR), claudin 7 (CLND7) and epithelial cell adhesion molecule (EPCAM) with the clinical indices and to reveal the diagnostic importance of these biomarkers in canine mammary tumours (CMTs). Peripheral blood (PB) samples were collected from 45 bitches (group MT) which had single mass with malignant epithelial tumours and 9 healthy bitches (group H). Real time PCR (rt-PCR) was performed to determine the expression levels of EGFR, CLDN7, and EPCAM. Mean values of EGFR and CLDN7 expressions were significantly higher in group MT compared to group H (P < 0.01 and P < 0.001, respectively). The expression level of CLDN7 was positively correlated with EGFR and EPCAM (P < 0.001 and P < 0.05, respectively). The EPCAM expression was associated with increased tumour size (P < 0.05) and EPCAM tended to decrease in the presence of skin ulceration on tumour (P = 0.05). Furthermore, expression levels of EGFR in intact dogs were significantly higher compared to spayed dogs in group MT (P < 0.01). The EGFR expression was significantly higher in the presence of metastases (P < 0.05). Also, increased EGFR was determined in grade 2 compared to grade 1 (P < 0.05). In conclusion, these results show that EGFR, CLDN7, EPCAM markers are measureable in PB and they may provide valuable information about the clinical pathophysiology of CMT.en_US
dc.description.sponsorshipScientific Research Found of Istanbul University [31510]en_US
dc.description.sponsorshipThis study was summarized from the first author's PhD Thesis which was supported by the Scientific Research Found of Istanbul University with project number: 31510.en_US
dc.identifier.citation0
dc.identifier.doi10.2754/avb202190020191
dc.identifier.endpage200en_US
dc.identifier.issn0001-7213
dc.identifier.issn1801-7576
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85111588029
dc.identifier.scopusqualityQ3
dc.identifier.startpage191en_US
dc.identifier.urihttps://doi.org/10.2754/avb202190020191
dc.identifier.urihttps://hdl.handle.net/20.500.14517/1561
dc.identifier.volume90en_US
dc.identifier.wosWOS:000740749900009
dc.identifier.wosqualityQ4
dc.language.isoen
dc.publisherveterinarni A Farmaceuticka Univerzita Brnoen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectClaudin 7en_US
dc.subjectepidermal growth factor receptoren_US
dc.subjectepithelial cell adhesion moleculeen_US
dc.titleDetermining the expression levels of circulating tumour cell markers in canine mammary tumoursen_US
dc.typeArticleen_US
dspace.entity.typePublication
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