Determining the expression levels of circulating tumour cell markers in canine mammary tumours

dc.authorid Degirmencioglu, Sevgin/0000-0001-7243-3671
dc.authorid Inal Gultekin, Guldal/0000-0002-8313-6119
dc.authorid Uçmak, Zeynep Günay/0000-0003-2530-1291
dc.authorscopusid 57217061843
dc.authorscopusid 36632450400
dc.authorscopusid 54580884800
dc.authorscopusid 25632114400
dc.authorscopusid 6603287153
dc.authorscopusid 58570213500
dc.authorwosid GUVENC, KAZIM/GQZ-4338-2022
dc.authorwosid Degirmencioglu, Sevgin/ABI-3962-2020
dc.authorwosid degirmencioglu, sevgin/M-4010-2017
dc.authorwosid Inal Gultekin, Guldal/AAF-5392-2021
dc.authorwosid Uçmak, Zeynep Günay/AAG-7917-2020
dc.contributor.author Ucmak, Zeynep Gunay
dc.contributor.author Kahraman, Ozlem Timirci
dc.contributor.author Gultekin, Guldal Inal
dc.contributor.author Degirmencioglu, Sevgin
dc.contributor.author Yaylim, Ilhan
dc.contributor.author Guvenc, Kazim
dc.date.accessioned 2024-05-25T11:42:15Z
dc.date.available 2024-05-25T11:42:15Z
dc.date.issued 2021
dc.department Okan University en_US
dc.department-temp [Ucmak, Zeynep Gunay; Guvenc, Kazim] Istanbul Univ Cerrahpasa, Dept Obstet & Gynaecol, Fac Vet Med, Istanbul, Turkey; [Kahraman, Ozlem Timirci; Yaylim, Ilhan] Istanbul Univ, Aziz Sancar Inst Expt Med, Dept Mol Med, Istanbul, Turkey; [Gultekin, Guldal Inal] Istanbul Okan Univ, Dept Physiol, Fac Med, Istanbul, Turkey; [Degirmencioglu, Sevgin] Istanbul Arel Univ, Dept Biochem, Fac Med, Istanbul, Turkey en_US
dc.description Degirmencioglu, Sevgin/0000-0001-7243-3671; Inal Gultekin, Guldal/0000-0002-8313-6119; Uçmak, Zeynep Günay/0000-0003-2530-1291 en_US
dc.description.abstract Detection of the circulating tumour cells (CTC) in dogs with a mammary tumour is a useful tool to reveal the micrometastases long before metastases are recognised clinically. The aim of this study was to evaluate the association of the epidermal growth factor receptor (EGFR), claudin 7 (CLND7) and epithelial cell adhesion molecule (EPCAM) with the clinical indices and to reveal the diagnostic importance of these biomarkers in canine mammary tumours (CMTs). Peripheral blood (PB) samples were collected from 45 bitches (group MT) which had single mass with malignant epithelial tumours and 9 healthy bitches (group H). Real time PCR (rt-PCR) was performed to determine the expression levels of EGFR, CLDN7, and EPCAM. Mean values of EGFR and CLDN7 expressions were significantly higher in group MT compared to group H (P < 0.01 and P < 0.001, respectively). The expression level of CLDN7 was positively correlated with EGFR and EPCAM (P < 0.001 and P < 0.05, respectively). The EPCAM expression was associated with increased tumour size (P < 0.05) and EPCAM tended to decrease in the presence of skin ulceration on tumour (P = 0.05). Furthermore, expression levels of EGFR in intact dogs were significantly higher compared to spayed dogs in group MT (P < 0.01). The EGFR expression was significantly higher in the presence of metastases (P < 0.05). Also, increased EGFR was determined in grade 2 compared to grade 1 (P < 0.05). In conclusion, these results show that EGFR, CLDN7, EPCAM markers are measureable in PB and they may provide valuable information about the clinical pathophysiology of CMT. en_US
dc.description.sponsorship Scientific Research Found of Istanbul University [31510] en_US
dc.description.sponsorship This study was summarized from the first author's PhD Thesis which was supported by the Scientific Research Found of Istanbul University with project number: 31510. en_US
dc.identifier.citationcount 0
dc.identifier.doi 10.2754/avb202190020191
dc.identifier.endpage 200 en_US
dc.identifier.issn 0001-7213
dc.identifier.issn 1801-7576
dc.identifier.issue 2 en_US
dc.identifier.scopus 2-s2.0-85111588029
dc.identifier.scopusquality Q3
dc.identifier.startpage 191 en_US
dc.identifier.uri https://doi.org/10.2754/avb202190020191
dc.identifier.uri https://hdl.handle.net/20.500.14517/1561
dc.identifier.volume 90 en_US
dc.identifier.wos WOS:000740749900009
dc.identifier.wosquality Q4
dc.language.iso en
dc.publisher veterinarni A Farmaceuticka Univerzita Brno en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.scopus.citedbyCount 0
dc.subject Claudin 7 en_US
dc.subject epidermal growth factor receptor en_US
dc.subject epithelial cell adhesion molecule en_US
dc.title Determining the expression levels of circulating tumour cell markers in canine mammary tumours en_US
dc.type Article en_US
dc.wos.citedbyCount 0

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