Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy

dc.authorid Gomez, Enrique/0000-0001-9037-9058
dc.authorid Llamosas, Marta Muñoz/0000-0002-9832-3523
dc.authorid Correia-Álvarez, Eva/0000-0002-8677-9822
dc.authorid Fernández-González, Alfonso/0000-0001-6240-2222
dc.authorid DIEZ, CARMEN/0000-0001-6909-7814
dc.authorid Uyar, Asli/0000-0002-7913-1083
dc.authorid Caamano, Jose Nestor/0000-0001-6449-5784
dc.authorscopusid 22135300300
dc.authorscopusid 55664907400
dc.authorscopusid 55614943000
dc.authorscopusid 13205557700
dc.authorscopusid 17934199900
dc.authorscopusid 57224427407
dc.authorscopusid 6701473546
dc.authorwosid Trigal, Beatriz/K-1972-2012
dc.authorwosid Gomez, Enrique/A-5604-2008
dc.authorwosid Llamosas, Marta Muñoz/A-7667-2008
dc.authorwosid Carrocera, Susana/H-7475-2015
dc.authorwosid Correia-Álvarez, Eva/GVS-6424-2022
dc.authorwosid Fernández-González, Alfonso/I-2720-2015
dc.authorwosid Caamano, Jose Nestor/L-3105-2017
dc.contributor.author Munoz, Marta
dc.contributor.author Uyar, Asli
dc.contributor.author Correia, Eva
dc.contributor.author Ponsart, Claire
dc.contributor.author Guyader-Joly, Catherine
dc.contributor.author Martinez-Bello, Daniel
dc.contributor.author Gomez, Enrique
dc.date.accessioned 2024-05-25T11:23:22Z
dc.date.available 2024-05-25T11:23:22Z
dc.date.issued 2014
dc.department Okan University en_US
dc.department-temp [Munoz, Marta; Correia, Eva; Diez, Carmen; Caamano, Jose Nestor; Trigal, Beatriz; Carrocera, Susana; Martin, David; Gomez, Enrique] Ctr Biotecnol Anim SERIDA, Gijon 33394, Asturias, Spain; [Uyar, Asli; Seli, Emre] Yale Univ, Sch Med, Dept Obstet Gynecol & Reprod Sci, New Haven, CT 06520 USA; [Uyar, Asli] Okan Univ, Dept Comp Engn, TR-34959 Istanbul, Turkey; [Ponsart, Claire; Marquant-Le Guienne, Brigitte] UNCEIA, Dept Res & Dev, F-94704 Maisons, Alfort, France; [Guyader-Joly, Catherine] UNCEIA, Stn Expt, F-38300 Chateauvillain, France; [Martinez-Bello, Daniel] UTE Bos Lugar Bos, La Coruna 15640, Spain; [Martinez-Bello, Daniel] EMBRIOVET SL, La Coruna 15165, Spain; [Fernandez-Gonzalez, Alfonso] Univ Oviedo, Serv Cient Tecn Oviedo, E-33006 Oviedo, Asturias, Spain; [Humblot, Patrice] Swedish Univ Agr Sci SLU, Dept Clin Sci, S-75007 Uppsala, Sweden en_US
dc.description Gomez, Enrique/0000-0001-9037-9058; Llamosas, Marta Muñoz/0000-0002-9832-3523; Correia-Álvarez, Eva/0000-0002-8677-9822; Fernández-González, Alfonso/0000-0001-6240-2222; DIEZ, CARMEN/0000-0001-6909-7814; Uyar, Asli/0000-0002-7913-1083; Ponsart, Claire/0000-0002-9633-4464; Caamano, Jose Nestor/0000-0001-6449-5784 en_US
dc.description.abstract We analyzed embryo culture medium (CM) and recipient blood plasma using Fourier transform infrared spectroscopy (FTIR) metabolomics to identify spectral models predictive of pregnancy outcome. Embryos collected on Day 6 from superovulated cows in 2 countries were individually cultured in synthetic oviduct fluid medium with BSA for 24 h before embryo transfer. Spent CM, blank controls, and plasma samples (Day 0 and Day 7) were evaluated using FTIR. The spectra obtained were analyzed. The discrimination capability of the classifiers was assessed for accuracy, sensitivity (pregnancy), specificity (nonpregnancy), and area under the ROC curve (AUC). Endpoints considered were Day 60 pregnancy and birth. High AUC was obtained for Day 60 pregnancy in CM within individual laboratories (France AUC = 0.751 +/- 0.039, Spain AUC = 0.718 +/- 0.024), while cumulative data decreased the AUC (AUC = 0.604 +/- 0.029). Predictions for CM at birth were lower than Day 60 pregnancy. Predictions with plasma at birth improved cumulative over individual results (Day 0: France AUC = 0.690 +/- 0.044; Spain AUC < 0.55; cumulative AUC = 0.747 +/- 0.032). Plasma generally predicted pregnancy and birth better than CM. These first results show that FTIR metabolomics could allow the identification of embryos and recipients with improved pregnancy viability, which may contribute to increasing the efficiency of selection schemes based on ET. en_US
dc.description.sponsorship Spanish Ministry of Economy and Competitiveness [AGL2012-37772]; FEDER; National Institute of Health (NIH), USA [Award R01HD059909]; COST Action [FA1201]; [MICINN-RYC08-03454]; [MEC-FPU-AP2009- 5265] en_US
dc.description.sponsorship The authors thank F. Goyache for valuable scientific comments. Project support was provided by the Spanish Ministry of Economy and Competitiveness, Project AGL2012-37772 and FEDER. Marta Munoz, Eva Correia, and Beatriz Trigal are supported by MICINN-RYC08-03454, MEC-FPU-AP2009- 5265, and Cajastur, respectively. Emre Seli is supported by Award R01HD059909 from the National Institute of Health (NIH), USA. The authors are members of the COST Action FA1201 Epiconcept: Epigenetics and Periconception Environment. en_US
dc.identifier.citationcount 27
dc.identifier.doi 10.1155/2014/608579
dc.identifier.issn 2314-6133
dc.identifier.issn 2314-6141
dc.identifier.pmid 24834432
dc.identifier.scopus 2-s2.0-84899998640
dc.identifier.scopusquality Q1
dc.identifier.uri https://doi.org/10.1155/2014/608579
dc.identifier.uri https://hdl.handle.net/20.500.14517/721
dc.identifier.volume 2014 en_US
dc.identifier.wos WOS:000335042500001
dc.institutionauthor Uyar A.
dc.language.iso en
dc.publisher Hindawi Ltd en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.scopus.citedbyCount 28
dc.subject [No Keyword Available] en_US
dc.title Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy en_US
dc.type Article en_US
dc.wos.citedbyCount 29

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