Qualitative and quantitative assesment of initial bacterial plaque formation on different prosthetic restorative materials

Abstract

Aim: The aim of this study was to determine the structure ofbacterial plaque qualitatively and quantitatively and to evaluate the effect of restorative material variation on pellicle composition and bacterial adhesion.Materials and Method: Titanium, Au-Ag-Pd alloy, Ni-Cr alloy, an all-ceramic system and a feldspathic porcelain systemwere used to evaluate bacterial adhesion and plaque composition characteristics. Acquired pellicle was analyzed by usingsodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS-PAGE). Single colony isolated groups on samples wereidentified by using BBL-Crystal System. Statistical analysiswas performed by using Kruskal Wallis Analysis of Variance(ANOVA).Results: Acquired pellicle analysis by SDS-PAGE revealedamylase content for each group except feldspathic porcelain.Significant qualitative and quantitative differences werefound among the materials for bacterial plaque content. Microorganism types varied most for feldspathic porcelain surface. The least variety of microorganisms were found on Cr-Ni.The lowest adhesion was observed on feldspathic porcelain.Conclusion: Initial bacterial plaque shows material specificdifferences for compositional structure. Bacterial adhesionfor each material shows internal compositional variations.Streptococci were found to be most revealed species for allmaterials studied.