Acar, Ozge KarabiyikNozhatzadeh, Gulcin DelalTuncer, AlperenKose, Gamze TorunHacihasanoglu, EzgiSahin, FikrettinAysan, ErhanGenetik ve Biyomühendislik / Genetic and Bio-Engineering2024-05-252024-05-25202220025-79741536-596410.1097/MD.00000000000320092-s2.0-85143560059https://doi.org/10.1097/MD.0000000000032009https://hdl.handle.net/20.500.14517/941Karabiyik Acar, Ozge/0000-0003-2697-6477; Hacihasanoglu, Ezgi/0000-0002-6661-1002; NOZHATZADEH, GULCIN DELAL/0000-0002-8532-7349; Sahin, Fikrettin/0000-0003-1503-5567Background:Parathyroid-like cells were aimed to be developed using cells isolated from thyroid since their embryological origins are the same. Method:Activin A and sonic hedgehog (Shh) are the proteins used in differentiation (dif) medium. Parathyroid and thyroid cells were cultured in a 3-dimensional environment and divided into five groups: thyroid standard (st) medium, thyroid dif medium, parathyroid st medium, thyroid-parathyroid co-culture st medium, and thyroid-parathyroid co-culture dif medium. Throughout 28 days of incubation, groups were investigated by carrying out the live dead assay, confocal microscopy, real-time PCR, immunohistochemistry and biochemical assays. Results:Thyroid-parathyroid co-culture cells grown in dif medium exhibited upregulated expressions of parathormone (PTH) (5.1-fold), PTH1R (3.6-fold), calcium sensing receptor (CaSR) (8.8-fold), and loss of thyroid-specific thyroid transcription factor 1 (TTF1) expression when compared to the thyroid st medium group. PTH secretion decreased by 35% in the parathyroid st medium group and 99.9% in the thyroid-parathyroid co-culture st medium group but decreased only 3.5% in the thyroid-parathyroid co-culture dif medium group on day 28. Conclusion:Using Activin A and Shh proteins, thyroid stem/progenitor cells were differentiated to parathyroid-like cells successfully in a co-culture environment. A potentially effective novel method for cell differenatiation is co-culture of cells having the same embryological origin.eninfo:eu-repo/semantics/openAccessco-culturedifferentiationparathormone (PTH)parathyroidstem cellthyroidArticleQ3Q210148WOS:00089550260003936482540