Meme kanseri hücre hattında kakule ile omega-3'ün (DHA) apoptoza etkisinin araştırılması
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2023
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Amaç: Çalışmanın amacı kakule ve Omega-3 (DHA)'ün MCF-7 insan meme kanserinde indüklenen, meme kanser modelinde oluşabilecek apoptotik ve antiapoptotik yol açıp açmadığını araştırmak ve Kakule ile Omega-3 (DHA)'ün koruyucu etkinliğini incelemek ve belirlemektir. Gereç ve Yöntem: Kakule ekstresi hazırlandı ve DHA bir firmadan temin edildi. MCF-7 hücreleri kakule ve DHA'nın değişen konsantrasyonlarıyla 24 ve 48 saat inkübe edildi. MTT testi ile hücre canlılıkları belirlendi. Ayrıca kakule ve DHA'nın farklı konsantrasyonlarda kombinasyonlarıyla da MCF-7 hücreleri inkübe edildi ve MTT testi ile hücre canlılıkları belirlendi. BAX, p53, sitokrom-C ve CAPN-1 düzeyleri ELISA yöntemi ile analiz edildi. Bulgular: Bu çalışmada, MCF-7 hücreleri üzerinde kakule, DHA ve kakule+DHA'nın etkisi incelenmiştir. 24 ve 48 saat sonunda, kakule, DHA ve kakule+DHA'nın hücre canlılığını anlamlı düzeyde azalttığı belirlenmiştir. Kakule dozu 24 saatlik 200 μM olarak belirlenirken, DHA dozu 24 saatlik 50 μM olarak belirlenmiştir. Kontrol grubu ile kıyaslandığında, kakule200+DHA50 kombinasyonunda 24 saatlik IC50 değeri 104,1 μM olarak saptanmıştır. BAX, p53, sitokrom-C ve CAPN1 konsantrasyonları açısından, kontrol grubu ile tedavi grupları arasında istatistiksel olarak anlamlı farklılıklar tespit edilmiştir (p<0,001). Bu sonuçlar, kakule, DHA ve kakule+DHA'nın MCF-7 hücrelerindeki canlılığı azaltabileceğini ve hücre ölümüne katkıda bulunabileceğini göstermektedir. Sonuç: Kakule ve DHA, MCF-7 hücrelerinde her yolakta apoptotik etkiye sahip değildir fakat belirli yolaklarda apoptozun sağlanmasına destek olabilir. Çeşitli bitkisel ekstrelerin ve besin takviyelerinin MCF-7 hücre hattında apoptoz üzerindeki etkisinin daha geniş kapsamlı bir şekilde araştırılması ve bu konuda literatürde daha fazla çalışmanın yer alması gereklidir. Hayvan deneyleri ve klinik çalışmaların yapılması, bu bileşenlerin ve diğer bitkisel ekstrelerin meme kanseri tedavisindeki etkinliğini ve güvenliğini değerlendirmek için gereklidir. Hayvan deneyleri, in vivo koşullarda bileşenlerin etkilerini daha gerçekçi bir şekilde değerlendirebilirken, klinik çalışmalar insanlarda tedaviye yanıtı ve olası yan etkileri değerlendirmek için önemlidir. Bu tür çalışmalar, bitkisel ekstrelerin ve besin takviyelerinin meme kanseri tedavisindeki potansiyel rolünü daha iyi anlamamıza yardımcı olacak ve hastalara daha etkili ve güvenli tedavi seçenekleri sunma potansiyelini taşıyacaktır. Anahtar Kelimeler: Meme kanseri, MCF-7, Kakule, ω-3, DHA, Apoptoz
Objective: This study aimed to investigate whether cardamom and Omega-3 (DHA) induce apoptotic and anti-apoptotic pathways in MCF-7 human breast cancer cells and to examine and determine the protective effect of cardamom and Omega-3 (DHA) in breast cancer models. Materials and Methods: cardamom extract was prepared, and DHA was obtained from a company. MCF-7 cells were incubated with varying concentrations of cardamom and DHA for 24 and 48 hours. Cell viability was determined using the MTT assay. Additionally, MCF-7 cells were incubated with different concentrations of cardamom and DHA in combination, and cell viability was determined using the MTT assay. BAX, p53, cytochrome-C, and CAPN-1 levels were analyzed using the ELISA method. Results: This study examined the effects of cardamom, DHA, and cardamom+DHA on MCF-7 cells. It was determined that cardamom, DHA, and cardamom+DHA significantly reduced cell viability at 24 and 48 hours. The cardamom dose was determined to be 200 μM for 24 hours, while the DHA dose was determined to be 50 μM for 24 hours. Compared to the control group, the 24-hour IC50 value in the cardamom200+DHA50 combination was 104.1 μM. There were statistically significant differences between the control and treatment groups regarding BAX, p53, cytochrome-C, and CAPN1 concentrations (p<0.001). These results indicate that cardamom, DHA, and cardamom+DHA can reduce cell viability and contribute to cell death in MCF-7 cells. Conclusion: cardamom and DHA do not have apoptotic effects on every pathway in MCF-7 cells. However, they can achieve apoptosis in specific ways. Further research is needed to investigate the impact of various herbal extracts and dietary supplements on apoptosis in the MCF-7 cell line in a more comprehensive manner, and more studies are required in the literature on this subject. Animal experiments and clinical trials are necessary to evaluate the efficacy and safety of these components and other herbal extracts in breast cancer treatment. Animal experiments can more realistically determine the effects of components under in vivo conditions. At the same time, clinical trials are essential to evaluate the response to treatment and possible side effects in humans. Such studies will help us better understand the potential role of herbal extracts and dietary supplements in breast cancer treatment and have the potential to provide patients with more effective and safe treatment options. Keywords: Breast cancer, MCF-7, Cardamom, ω-3, DHA, Apoptosis.
Objective: This study aimed to investigate whether cardamom and Omega-3 (DHA) induce apoptotic and anti-apoptotic pathways in MCF-7 human breast cancer cells and to examine and determine the protective effect of cardamom and Omega-3 (DHA) in breast cancer models. Materials and Methods: cardamom extract was prepared, and DHA was obtained from a company. MCF-7 cells were incubated with varying concentrations of cardamom and DHA for 24 and 48 hours. Cell viability was determined using the MTT assay. Additionally, MCF-7 cells were incubated with different concentrations of cardamom and DHA in combination, and cell viability was determined using the MTT assay. BAX, p53, cytochrome-C, and CAPN-1 levels were analyzed using the ELISA method. Results: This study examined the effects of cardamom, DHA, and cardamom+DHA on MCF-7 cells. It was determined that cardamom, DHA, and cardamom+DHA significantly reduced cell viability at 24 and 48 hours. The cardamom dose was determined to be 200 μM for 24 hours, while the DHA dose was determined to be 50 μM for 24 hours. Compared to the control group, the 24-hour IC50 value in the cardamom200+DHA50 combination was 104.1 μM. There were statistically significant differences between the control and treatment groups regarding BAX, p53, cytochrome-C, and CAPN1 concentrations (p<0.001). These results indicate that cardamom, DHA, and cardamom+DHA can reduce cell viability and contribute to cell death in MCF-7 cells. Conclusion: cardamom and DHA do not have apoptotic effects on every pathway in MCF-7 cells. However, they can achieve apoptosis in specific ways. Further research is needed to investigate the impact of various herbal extracts and dietary supplements on apoptosis in the MCF-7 cell line in a more comprehensive manner, and more studies are required in the literature on this subject. Animal experiments and clinical trials are necessary to evaluate the efficacy and safety of these components and other herbal extracts in breast cancer treatment. Animal experiments can more realistically determine the effects of components under in vivo conditions. At the same time, clinical trials are essential to evaluate the response to treatment and possible side effects in humans. Such studies will help us better understand the potential role of herbal extracts and dietary supplements in breast cancer treatment and have the potential to provide patients with more effective and safe treatment options. Keywords: Breast cancer, MCF-7, Cardamom, ω-3, DHA, Apoptosis.
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Beslenme ve Diyetetik, Biyokimya, Nutrition and Dietetics, Biochemistry, Onkoloji, Oncology
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74