Histological Evaluation of Algan Hemostatic Agent's Effect in a Rat Experimental Spleen Injury Model

dc.authorscopusid 59503547500
dc.authorscopusid 59503715000
dc.authorscopusid 55910059600
dc.authorscopusid 59503801600
dc.authorscopusid 59503566400
dc.contributor.author Şener Akçora, D.
dc.contributor.author Mavuş, D.
dc.contributor.author Şeker, U.
dc.contributor.author Yildiz, G.
dc.contributor.author Gökçe, K.
dc.date.accessioned 2025-02-17T18:49:43Z
dc.date.available 2025-02-17T18:49:43Z
dc.date.issued 2024
dc.department Okan University en_US
dc.department-temp Şener Akçora D., Department of Histology and Embryology, Faculty of Medicine, Marmara University, İstanbul, Turkey; Mavuş D., Faculty of Medicine, Istanbul Okan University, İstanbul, Turkey; Şeker U., Department of Histology and Embryology, Faculty of Medicine, Mardin Artuklu University, Mardin, Turkey; Yildiz G., Faculty of Medicine, Bahcesehir University, İstanbul, Turkey; Gökçe K., Department of General Surgery, Faculty of Medicine, Istanbul Okan University, İstanbul, Turkey en_US
dc.description.abstract Uncontrolled hemorrhage may result from injuries to the parenchym and splenic capsule. Hemostatic material applications are among the methods used to prevent spleen parenchymal hemorrhage. Algan Hemostatic Agent (AHA) is a standardized mixture of six distinct herbs that are capable of hemostasis, either individually or in combination. Aim of this study was to investigate efficiency of AHA in bleeding control in experimental spleen injury model, and to evaluate its histopathological effects and IL-1β, TNF-α, and Bax expressions in tissue. Twenty-four Wistar albino female rats, aged 10-12 weeks, were randomly separated into three groups (n=8); control, AHA-liquid, and AHA-powder. After surgery physiological saline, liquid and powder were applied to the injured area for twenty seconds. On the tenth postoperative day all rats’ spleens were removed for histopathological and immunohistochemical analysis. The AHA-liquid group demonstrated more efficacy in controlling hemorrhage than the AHA powder group after both the initial and subsequent applications. Parenchyma of the spleen was intact, and a thin capsule was detected in the liquid group. In the powder group, thick granulation tissue was observed along with acute lymphocyte and neutrophil infiltration. Expressions of IL-1 β and TNF-α were mild in control and AHA-liquid groups and intense in AHA-powder groups. Similar Bax protein expression was detected in all groups. Current study demonstrated that liquid form of AHA was more effective in reducing local bleeding and inflammation in spleen tissue. Therefore, liquid form could be preferred in animal experiments and clinics as a rapid, safe, and effective agent for organ injury. © 2024 Ondokuz Mayis Universitesi. All rights reserved. en_US
dc.identifier.citationcount 0
dc.identifier.doi 10.52142/omujecm.41.4.11
dc.identifier.endpage 753 en_US
dc.identifier.issn 1309-4483
dc.identifier.issue 4 en_US
dc.identifier.scopus 2-s2.0-85214331203
dc.identifier.scopusquality Q4
dc.identifier.startpage 746 en_US
dc.identifier.uri https://doi.org/10.52142/omujecm.41.4.11
dc.identifier.uri https://hdl.handle.net/20.500.14517/7681
dc.identifier.volume 41 en_US
dc.identifier.wosquality N/A
dc.language.iso en en_US
dc.publisher Ondokuz Mayis Universitesi en_US
dc.relation.ispartof Journal of Experimental and Clinical Medicine (Turkey) en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.scopus.citedbyCount 0
dc.subject Algan Hemostatic Agent en_US
dc.subject Bleeding en_US
dc.subject Histopathology en_US
dc.subject Immunohistochemistry en_US
dc.subject Rat en_US
dc.subject Splenectomy en_US
dc.title Histological Evaluation of Algan Hemostatic Agent's Effect in a Rat Experimental Spleen Injury Model en_US
dc.type Article en_US

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