TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14517/20

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Now showing 1 - 9 of 9

Citation Count: 2
Effects of tacrolimus on c-fos in hippocampus and memory performances in streptozotocin model of Alzheimer's disease of rats
(Tubitak Scientific & Technological Research Council Turkey, 2021) Altunkaynak, Berrin Zühal; Altunkaynak, Berrin Zuhal; DelIbas, Burcu; Histoloji ve Embriyoloji / Histology and Embriology
Background/aim: Calcineurin, an inhibitor of calcium dependent phosphatase is highly presented in a brain of an Alzheimer's disease. Aging brain gets more sensitive to hyperactivation of calcineurin, and this event causes tau neurofibrillary plaque accumulation, which is one of the outcomes of this disease. The regions of hippocampus are much effected from the results of this process. Our hypothesis is that a calcineurin inhibitor, tacrolimus, could prevent the accumulation and the decrease of the neuronal cells. Therefore, this immunosuppressive drug could be a candidate for an early treatment of Alzheimer disease. Materials and methods: Fifteen male Wistar albino rats were divided to three groups; control, Alzheimer, and Alzheimer+Tacrolimus. The Alzheimer group received an injection of streptozotocin intracerebroventricularly for the purpose of modelling the disease via generating free radicals leading a cognitive impairment. Alzheimer+Tacrolimus group first received an oral drug, a calcineurin inhibitor for 10 days afterwards prepared for the model as same as the Alzheimer group received. Finally, all groups performed the Morris water maze test for four days then sacrificed. For the aim of counting neurons in the hippocampus stereological methods, as well as for an evaluation of cellular response to stress in dentate gyrus, a c-Fos immunohistochemistry was performed. Results: According to the probe trial of Morris water maze test, the latency time was dramatically higher at both Alzheimer and Alzheimer+Tacrolimus group (p < 0.01). We confirmed these results with our stereology data. The results from stereology technique indicate that there was a neuronal decrease at the hippocampus regions in Alzheimer and Alzheimer+Tacrolimus group. Our outcomes from immunohistochemical data showed a significant increase in the number of c-Fos-positive cells in Alzheimer group when comparing with Alzheimer+Tacrolimus group (p < 0.001). Conclusion: There was none preventive effect for neuronal loss in the hippocampus under the effect of tacrolimus drug according to stereological results. However, tacrolimus administration may have reduced cellular stress and cell damage.
Citation Count: 2
Evaluation and Categorization of The Fishing Ports with a Fuzzy Spatial Multi Criteria Approach: The Case of Turkey
(Central Fisheries Research inst, 2017) Onden, Ismail; Samasti, Mesut; Canci, Metin; Eldemir, Fahrettin; Aktel, Abdullah
Fishing ports are the vital constituent of the fishery industry of Turkey. With governmental contribution, the number of the fishing ports reached to 366 alongside of the shores and the collected fish volume has been in increasing trend. However, there are differences in the location characteristics and technical infrastructure so that each facility's success level is measured differently with convenient parameters. To increase the performance of fishing ports, for their better utilization, and also to understand which of them can be transformed to regional centers a classification is needed. With the transformation to the regional centers, i.e. the infrastructure improvements of the facilities and providing multiple services such as tourism and transportation activities; the efficiency of the ports can be increased. In this paper, a classification methodology is developed and it is tested. While applying the methodology, expert workshops are carried out to represent current fishery environment in Turkey. In the meetings, decision criteria are discussed by participating over 200 experts in the field. f-AHP and GIS/Spatial Analysis is used to analyze spatial suitability. Results of the study showed that the methodology is capable of dealing with spatial and non-spatial characteristics of the data-set and determine the convenient alternatives.
Citation Count: 1
Identification of novel TUBB1 variants in patients with macrothrombocytopenia
(Tubitak Scientific & Technological Research Council Turkey, 2021) Özkan, Didem; Waheed, Abdullah Abdul; Ozturk, Merve Tuzlakoglu; Oymak, Yesim; Tazebay, Uygar Halis; Akar, Nejat; Ozkan, Didem Torun; Tıbbi Laboratuvar Teknikleri / Medical Laboratory Techniques
Background/aim: Macrothrombocytopenia is an autosomal-dominant disorder characterized by increased platelet size and a decreased number of circulating platelets. The membrane skeleton and the link between actin filaments of the skeleton and microtubules, which consist of alpha and beta tubulin [including the tubulin beta-1 chain (TUBB1)] heterodimers, are important for normal platelet morphology, and defects in these systems are associated with macrothrombocytopenia. Materials and methods: In this study, we sequenced the exons of the TUBB1 gene using DNA isolated from the peripheral blood samples of healthy controls (n = 47) and patients with macrothrombocytopenia (n = 37) from Turkey. The TUBB1 expression levels in fractioned blood samples from patients and healthy controls were analyzed by RT-qPCR and Western blot. Microtubule organization of the platelets in the peripheral blood smears of patients, and in mutant TUBB1-transfected HeLa cells, were analyzed by immunofluorescence staining. Results: A new TUBB1 c.803G > T (p.T178T) variant was detected in all of the control and patient samples. Importantly, we found 3 new heterozygous TUBB1 variants predicting amino acid substitutions: G146R (in 1 patient), E123Q (in 1 patient), and T274M (in 4 patients); the latter variant was associated with milder thrombocytopenia in cancer patients treated with paclitaxel. Ectopic expression of TUBB1 T274M/R307H variant in HeLa cells resulted in irregular microtubule organization. Conclusion: Further clinical and functional studies of the newly identified TUBB1 variants may offer important insights into their pathogenicity in macrothrombocytopenia.
Citation Count: 1
Ideological process of shopping malls within the framework of ‘glocalization’
(2013) Akıncı, Güliz Muğan
Kent yaşamının anlamının değişmesiyle birlikte şehirler tüketim olgusunun ana merkezleri haline gelirken, alışveriş merkezleri de modern şehirlerin önemli işaretlerinden biri haline geldi. Kentleşme sürecindeki küresel eğilimler alışveriş merkezlerini kent deneyiminin merkezi durumuna getirdi. Fiziksel özelliklerinin avantajlarını da kullanarak alışveriş merkezleri kent içerisinde stratejik noktalara konumlandırılıp tüketicilerin dikkatini çekerek, onları baştan çıkarmak üzere tasarlanmışlardır. Bu çalışmanın amacı, kapitalizmin yapısal değişikliklerine vurgu yaparak, alışveriş merkezleri sure geldiği varsayılan ideolojik süreci değerlendirmektir. Bunun yanı sıra, bu çalışma, alışveriş merkezlerinin küresel ve homojen yapısının nasıl fordizm sonrası kapitalist söylemi daha yerel, politik ve sosyal etkilerle bir araya getiren ‘glokalleşme’ sürecine doğru yol aldığını sorgular. Bu çerçevede, kapitalist toplumlardaki hakim ideoloji tezinin sınırlılıkları küreselleşme teması etrafında incelenme ve analiz edilme fırsatı bulur. Bu analiz için NicholasAbercrombie’nin “Popular CultureandIdeologicalEffects” [Popüler Kültür ve İdeolojik Etkiler] adlı makalesi ve hâkim ideoloji eleştirileri temel kaynak olarak kullanılacaktır.
Citation Count: 1
In vitro tooth-shaped scaffold construction by mimicking late bell stage
(Tubitak Scientific & Technological Research Council Turkey, 2020) Yalçın Ülker, Gül Merve; Yalcin Ulker, Gul Merve; Cumbul, Alev; Uslu, Unal; Yilmaz, Sahin; Bozkurt, Batuhan Turhan; Sahin, Fikrettin; Ağız,Diş ve Çene Cerrahisi / Oral, Dental and Maxillofacial Surgery
Neogenesis of osseous and ligamentous interfacial structures is essential for the regeneration of large oral or craniofacial defects. However, current treatment strategies are inadequate in renewing supporting tissues of teeth after trauma, chronic infections or surgical resection. Combined use of 3D scaffolds with stem cells became a promising treatment option for these injuries. Matching different scaffolding materials with different tissues can induce the correct cytokines and the differentiation of cells corresponding to that particular tissue. In this study, a hydroxyapatite (HA) based scaffold was used together with human adipose stem cells (hASCs), human bone marrow stem cells (hBMSCs) and gingival epithelial cells to mimic human tooth dentin-pulp-enamel tissue complexes and model an immature tooth at the late bell stage in vitro. Characteristics of the scaffold were determined via SEM, FTIR, pore size and density measurements. Changes in gene expression, protein secretions and tissue histology resulting from cross-interactions of different dental tissues grown in the system were shown. Classical tooth tissues such as cementum, pulp and bone like tissues were formed within the scaffold. Our study suggests that a HA-based scaffold with different cell lineages can successfully mimic early stages of tooth development and can be a valuable tool for hard tissue engineering.
Citation Count: 0
Obstructive sleep apnea syndrome is associated with impaired pulmonary artery distensibility and right ventricular systolic dysfunction
(Turkiye Klinikleri Journal of Medical Sciences, 2018) Çetin,S.; Vural,M.G.; Firat,H.; Akdemir,R.
Background/aim: We investigated whether obstructive sleep apnea syndrome (OSAS) has any impact on pulmonary artery distensibility (PAD) and right ventricular (RV) function. Materials and methods: Subjects were categorized according to apnea–hypopnea index (AHI) as follows: controls (n = 17 and AHI < 5), mild-to-moderate OSAS (n = 22 and AHI = 5–30), and severe OSAS (n = 29 and AHI > 30). All subjects underwent transthoracic echocardiography after polysomnography to assess PAD and RV function. PAD was recorded as M-Mode trace of the right pulmonary artery and was defined as (PAmax –PAmin/PAmin) × 100. S’ was measured by means of TDI of the lateral annulus of the RV using apical four-chamber view. Results: Patients with severe OSAS demonstrated impaired RV longitudinal systolic function (S’) compared to the other groups (P < 0.05). Impaired pulmonary vasculature elastic properties as reflected by decreased PAD were more prevalent in severe OSAS (26.2 ± 5.7%) compared to the controls (29.9 ± 4.6%; P < 0.05) and mild-to-moderate OSAS (29.0 ± 4.1%; P < 0.05). An inverse relation between PAD (P < 0.05), RV myocardial performance index (MPI) (P < 0.05), and AHI was demonstrated. S’ also correlated with PAD (P < 0.05). Conclusion: PAD is a significant tool to evaluate pulmonary vasculature stiffening and is well correlated with disease severity in OSAS. Further, impaired PAD may lead to RV systolic dysfunction. © TÜBİTAK.
Citation Count: 0
Percutaneous transhepatic management of biliary strictures in patients with dysfunctioning plastic biliary endoprostheses
(Tubitak Scientific & Technological Research Council Turkey, 2022) Oguslu, Umut; Danisan, Gurkan; Gumus, Burcak
Background/aim: To evaluate the safety and outcomes of percutaneous transhepatic management of dysfunctioning plastic biliary endoprostheses (PBE) in patients with benign/malign biliary strictures. Materials and methods: Twenty-nine patients (22 men, 7 women; mean age of 60.7 (range 33-88) years) diagnosed with dysfunctioning PBE were included. Percutaneous transhepatic biliary drainage and subsequent PBE dislodgment into the bowel were performed in all cases. Patient demographics, etiology of the biliary stricture, indication, technical success, complications, and clinical outcomes were gleaned from medical records. Results: Seventeen patients had malignant strictures, while 12 patients had benign conditions. A total of 36 PBE (33 straight, 3 double-J) were treated. Six patients had more than one PBE. Successful dislodgement of the PBE was achieved in 28 (96.6%) of the cases. Monorail threading was performed in 8 cases while dislodgement by balloon friction was utilized in 21 patients. There was no statistical significance between benign and malignant biliary strictures regarding dislodgement duration (p = 0.080). No major complication was encountered. Thirteen minor complications in 10 patients including abdominal pain (n = 8) and mild hemobilia (n = 5) were observed and treated conservatively. Uneventful passage of the PBE was reported by all patients with technical success. Conclusion: Percutaneous transhepatic methods aid as a reasonable alternative in the treatment of benign and malignant biliary strictures in patients with dysfunctioning PBE when endoscopic approaches fail or are not eligible.
Citation Count: 0
Retrospective analysis of effectiveness of fingolimod in real life setting in Turkey (REFINE)
(Turkiye Klinikleri, 2023) Tuncer,A.; Kürtüncü,M.; Terzi,M.; Uygunoğlu,U.; Göncüoğlu,C.; Yüceyar,A.N.; Eraksoy,M.
Background/aim: During multiple sclerosis (MS) treatment different modes of action such as lateral (interferon beta to glatiramer acetate or glatiramer acetate to interferon beta) or vertical (interferon beta/glatiramer acetate to fingolimod) drug switch can be performed. This study aims to investigate the clinical effectiveness of switching from the first-line injectable disease modifying treatments (iDMTs) to fingolimod (FNG) compared to switching between first-line iDMTs. Materials and methods: This is a multicenter, observational and retrospective study of patients with relapsing-remitting MS who had lateral and vertical switch. The observation period included three key assessment time points (before the switch, at switch, and after the switch). Data were collected from the MS patients’ database by neurologists between January 2018 and June 2019. The longest follow-up period of the patients was determined as 24 months after the switch. Results: In 462 MS patients that were included in the study, both treatments significantly decreased the number of relapses during the postswitch 12 months versus preswitch one year while patients in the FNG group experienced significantly fewer relapses compared to iDMT cohort in the postswitch 12 months period. FNG cohort experienced fewer relapses than in the iDMT cohort within the postswitch 2 year. The mean time to first relapse after the switch was significantly longer in the FNG group. Conclusion: The present study revealed superior effectiveness of vertical switch over lateral switch regarding the improvement in relapse outcomes. Patients in the FNG cohort experienced sustainably fewer relapses during the follow-up period after the switch compared the iDMT cohort. Importantly, switching to FNG was more effective in delaying time to first relapse when compared with iDMTs. © TÜBİTAK.
Citation Count: 1
Spatial Analysis of Effective Coastal Land Use Policies for the Development of Amateur Marine in Turkey
(2018) İsmail ÖNDEN; Metin ÇANCI
Recreational fishing, sailing, activities involving boating are parts of the social life-style which family members practicesoutdoors as social activities throughout the counties where maritime is common. It is being observed that, these kinds ofactivities tend to improve in Turkey within the recent years for the reasons like the increase in the national income andacculturation. Consequently, in order for satisfying this demand, new mooring areas with reasonable costs are required. Inorder to fulfill the berthing and mooring needs of the vessels, cost efficient and environmental solution approaches are need. This article aims to develop a system offering for constitution of sustainable policies with regards to determination of boats berthing and mooring areas that are needed for amateur marine in Turkey. For that reason, geographic information system (GIS) based approaches are taken into consideration for spatial analysis, which is used to specify suggested facilities regarding any component that generates the system. The results showed that the suggested method successfully functions regarding the selection of facility location, and it has been detected that by the suggested system approach, it is possible to achieve new and higher capacities.

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