Alginate-Based Cell Encapsulation Using Different Crosslinker Elements

dc.authoridKarabiyik Acar, Ozge/0000-0003-2697-6477
dc.authorscopusid56491014000
dc.authorscopusid57997273700
dc.authorscopusid7004923875
dc.authorscopusid7003485202
dc.authorscopusid8614825000
dc.contributor.authorAcar, Ö.K.
dc.contributor.authorTuncer, A.A.
dc.contributor.authorŞahin, F.
dc.contributor.authorKöse, G.T.
dc.contributor.authorAysan, E.
dc.contributor.otherGenetik ve Biyomühendislik / Genetic and Bio-Engineering
dc.date.accessioned2024-10-15T20:20:22Z
dc.date.available2024-10-15T20:20:22Z
dc.date.issued2025
dc.departmentOkan Universityen_US
dc.department-tempAcar Ö.K., Department of Genetics and Bioengineering, Istanbul Okan University, Istanbul, 34959, Turkey, Department of Genetics and Bioengineering, Yeditepe University, Istanbul, 34755, Turkey; Tuncer A.A., Department of Genetics and Bioengineering, Yeditepe University, Istanbul, 34755, Turkey; Şahin F., Department of Genetics and Bioengineering, Yeditepe University, Istanbul, 34755, Turkey; Köse G.T., Department of Genetics and Bioengineering, Yeditepe University, Istanbul, 34755, Turkey; Aysan E., Department of General Surgery, Yeditepe University Hospital, Istanbul, 34718, Turkeyen_US
dc.description.abstractAlginate microcapsules are the most frequently used materials for cell transplantation. Different crosslinkers affect crosslinking affinity, which has a significant influence on microcapsule properties. The objective was to prepare in vitro microcapsules using calcium, barium, iron, manganese, nickel, and strontium as divalent cations to observe their potential for use in cell transplantation. Sodium alginate was added dropwise to the individually prepared crosslinkers to observe diffusion-based gelling. Alginate microcapsules were investigated regarding capsule stability, physiological properties, and cell viability. After 30 days of incubation, cell viability was greater than 90% for the cell-encapsulated microcapsules when crosslinked with CaCl2 and NiCl2. Viability decreased in the following order: CaCl2 > NiCl2 > BaCl2 > SrCl2 > MnCl2 > FeCl2. A compression test was performed to investigate the required force to deform 30% of microcapsules, and only MnCl2, FeCl2 (180 mM), and NiCl2 (50 mM) demonstrated higher resistance to the applied force than CaCl2. Except for the FeCl2 group, all cell-encapsulated microcapsules remained intact for 45 days. Potential sensitivities to CaCl2 during cell transplantation may compel alternative crosslinker usage, and our study revealed that NiCl2 and BaCl2 can be used as alternative crosslinkers to CaCl2 due to their high cell viability and consistent stability. © World Scientific Publishing Company.en_US
dc.description.woscitationindexScience Citation Index Expanded
dc.identifier.citationcount0
dc.identifier.doi10.1142/S0219519424500313
dc.identifier.issn0219-5194
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-105001063258
dc.identifier.scopusqualityQ4
dc.identifier.urihttps://doi.org/10.1142/S0219519424500313
dc.identifier.volume25en_US
dc.identifier.wosWOS:001318742100001
dc.identifier.wosqualityQ4
dc.institutionauthorAcar, Özge
dc.institutionauthorAcar, Özge
dc.language.isoen
dc.language.isoenen_US
dc.publisherWorld Scientificen_US
dc.relation.ispartofJournal of Mechanics in Medicine and Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.scopus.citedbyCount0
dc.subjectCrosslinkeren_US
dc.subjectDivalent Cationsen_US
dc.subjectHuman Dermal Fibroblasten_US
dc.subjectSodium Alginateen_US
dc.subjectTransplantationen_US
dc.titleAlginate-Based Cell Encapsulation Using Different Crosslinker Elementsen_US
dc.typeArticleen_US
dc.wos.citedbyCount0
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery43828c7d-a780-49c0-9e4f-82a39913e5c0
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relation.isOrgUnitOfPublication.latestForDiscovery2b8689c6-2a28-45db-b81c-7f828c944e77

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