Is melatonin, leptin or their combination more effective on oxidative stress and folliculogenesis in the obese rats?

dc.authoridtumentemur, gamze/0000-0002-3114-634X
dc.authorscopusid55898294200
dc.authorscopusid15063935100
dc.authorscopusid7403238396
dc.authorwosidtumentemur, gamze/HOC-6704-2023
dc.authorwosidKaplan, Suleyman/A-5396-2008
dc.contributor.authorAltunkaynak, Berrin Zühal
dc.contributor.authorAltunkaynak, Berrin Zuhal
dc.contributor.authorKaplan, Suleyman
dc.contributor.otherHistoloji ve Embriyoloji / Histology and Embriology
dc.date.accessioned2024-05-25T11:40:00Z
dc.date.available2024-05-25T11:40:00Z
dc.date.issued2020
dc.departmentOkan Universityen_US
dc.department-temp[Kirikci, Gamze] Acibadem Mehmet Ali Aydinlar Univ, Vocat Sch Hlth Serv, Istanbul, Turkey; [Altunkaynak, Berrin Zuhal] Okan Univ, Fac Med, Dept Histol & Embryol, Istanbul, Turkey; [Kaplan, Suleyman] Ondokuz Mayis Univ, Med Fac, Dept Histol & Embryol, Samsun, Turkeyen_US
dc.descriptiontumentemur, gamze/0000-0002-3114-634X;en_US
dc.description.abstractIn this study, we evaluated the effects of melatonin (Mel), leptin (Lep) or melatonin and leptin treatment on ovaries in control and obese rats. The animals were divided into control (NC), melatonin (NM), leptin (NL), melatonin?leptin (NML), obese (OC), obese?melatonin (OM), obese?leptin (OL), obese?melatonin?leptin (OML) groups. Body weights, peri-ovarian fat pads, volumetric parameters and numerical values of follicles were estimated. Also, the LH receptor (LHr) immune-positivity, catalase (CAT) and the myeloperoxidase (MPO) levels were determined. The body weight and peri-ovarian fat pads were significantly decreased following Mel (p?<?.05) treatment and, especially, Lep (p?<?.01) treatment. But, the ovarian weights were significantly increased following Lep (p?<?.05) and Mel (p?<?.01) treatment, in particular. The ovarian and cortex volume decreased in the OC group, and the cortex volume of the OC group was significantly higher than the Ob?+?Mel, Ob?+?Lep and Ob?+?Mel?+?Lep groups (p?<?.01). Besides, the volume of the cortex in the NL group was significantly higher than in the other groups (except for the NC group) (p?<?.01). Although, the total numbers of primordial and primary follicles in NC group were significantly higher than in the OC group (p?<?.001), the number of the primordial and primary follicles in OC group was significantly higher than in the OL (p?<?.05), OM (p?<?.05) and, especially, the OML groups (p?<?.001). Likewise, the number of the secondary follicles in the OML group was significantly less than that in the OC group (p?<?.05). The CAT and MPO activity of the OC group was significantly higher than in the NC group (p?<?.05) and also granulosa cell apoptosis had increased in obese rats; but it was decreased after Lep and Mel treatment. Otherwise, Lep and, in particular, Mel increased LHr positivity. We concluded that obesity could trigger abnormal ovarian function and polycystic ovary via inducing LHr apoptosis and suppressing ovarian folliculogenesis. Also, melatonin could be better for inhibition of apoptosis and modulation of folliculogenesis than leptin. These observations suggest that melatonin may act to reduce fertility in obese patients.Impact statement What is already known on this subject? Hormonal changes during reproductive cycle in obese women are particularly studied and there is not any study that evaluates the effects of melatonin and leptin, together. What the results of this study add? The study has shown that obese rats have increased granulosa cell apoptosis and MPO activities but melatonin and leptin reduces the apoptosis and inflammation. Moreover, the obesity decreased, but melatonin and leptin increased LHR immunoreactivity in both the granulosa and theca cells. What are of these findings for clinical practice and/or further research? The results suggest that leptin and melatonin could decrease excess body weight in obese persons. Also, these hormones modulate the ovarian turn-over by regulating developing follicles. Therefore, leptin and especially melatonin could be used as a supplement to ovulation therapy.en_US
dc.identifier.citation7
dc.identifier.doi10.1080/01443615.2019.1657816
dc.identifier.endpage127en_US
dc.identifier.issn0144-3615
dc.identifier.issn1364-6893
dc.identifier.issue1en_US
dc.identifier.pmid31625776
dc.identifier.scopus2-s2.0-85074323085
dc.identifier.scopusqualityQ3
dc.identifier.startpage116en_US
dc.identifier.urihttps://doi.org/10.1080/01443615.2019.1657816
dc.identifier.urihttps://hdl.handle.net/20.500.14517/1387
dc.identifier.volume40en_US
dc.identifier.wosWOS:000491387400001
dc.identifier.wosqualityQ4
dc.language.isoen
dc.publisherTaylor & Francis incen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBody mass indexen_US
dc.subjectweight lossen_US
dc.subjectovarian morphologyen_US
dc.subjectleptinen_US
dc.subjectmelatoninen_US
dc.subjectstereologyen_US
dc.titleIs melatonin, leptin or their combination more effective on oxidative stress and folliculogenesis in the obese rats?en_US
dc.typeArticleen_US
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery6322a75b-d2c9-4ab3-9e93-6d62d46b6331
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