Microencapsulation of parathyroid cells via electric field and non-surgical transplantation approach

dc.authorid Karabiyik Acar, Ozge/0000-0003-2697-6477
dc.authorid NOZHATZADEH, GULCIN DELAL/0000-0002-8532-7349
dc.authorid Hacihasanoglu, Ezgi/0000-0002-6661-1002
dc.authorscopusid 56491014000
dc.authorscopusid 36175380600
dc.authorscopusid 57219518809
dc.authorscopusid 57997165700
dc.authorscopusid 56462984800
dc.authorscopusid 57997273700
dc.authorscopusid 7003485202
dc.authorwosid Karabiyik Acar, Ozge/AAX-9366-2021
dc.authorwosid Hacihasanoglu, Ezgi/U-5919-2018
dc.contributor.author Acar, O. Karabiyik
dc.contributor.author Basoglu, H.
dc.contributor.author Kegin, M.
dc.contributor.author Nozhatzadeh, G. D.
dc.contributor.author Hacihasanoglu, E.
dc.contributor.author Tuncer, A. A.
dc.contributor.author Aysan, E.
dc.date.accessioned 2024-05-25T11:38:44Z
dc.date.available 2024-05-25T11:38:44Z
dc.date.issued 2023
dc.department Okan University en_US
dc.department-temp [Acar, O. Karabiyik; Nozhatzadeh, G. D.] Istanbul Okan Univ, Fac Engn & Nat Sci, Dept Genet & Bioengn, TR-34959 Istanbul, Turkiye; [Acar, O. Karabiyik; Kegin, M.; Nozhatzadeh, G. D.; Tuncer, A. A.; Sahin, F.; Kose, G. Torun] Yeditepe Univ, Fac Engn, Dept Genet & Bioengn, TR-34755 Istanbul, Turkiye; [Basoglu, H.] Karadeniz Tech Univ, Fac Med, Dept Biophys, TR-61080 Trabzon, Turkiye; [Kegin, M.] Hlth Sci Univ, Dept Gen Surg, GOP EAH, TR-34255 Istanbul, Turkiye; [Hacihasanoglu, E.] Yeditepe Univ, Fac Med, Dept Pathol, TR-34718 Istanbul, Turkiye; [Aysan, E.] Yeditepe Univ, Fac Med, Dept Gen Surg, TR-34718 Istanbul, Turkiye en_US
dc.description Karabiyik Acar, Ozge/0000-0003-2697-6477; NOZHATZADEH, GULCIN DELAL/0000-0002-8532-7349; Hacihasanoglu, Ezgi/0000-0002-6661-1002 en_US
dc.description.abstract Purpose Hypoparathyroidism is a rare disease with low PTH, mostly seen as a consequence of neck surgery. Current management is the prescription of calcium and vitamin D, but the definitive treatment is parathyroid allotransplantation, which frequently triggers an immune response, thus cannot achieve the expected success. To overcome this problem, encapsulation of allogeneic cells is the most promising method. By optimizing the standard alginate cell encapsulation technique with parathyroid cells under high-voltage application, the authors reduced the size of parathyroid-encapsulated beads and evaluated these samples in vitro and in vivo. Methods Parathyroid cells were isolated, and standard-sized alginate macrobeads were prepared without any electrical field application, while microbeads in smaller sizes (< 500 mu m), by the application of 13 kV. Bead morphologies, cell viability, and PTH secretion were evaluated in vitro for four weeks. For the in vivo part, beads were transplanted into Sprague-Dawley rats, and after retrieval, immunohistochemistry and PTH release were evaluated in addition to the assessment of cytokine/chemokine levels. Results The viability of parathyroid cells in micro- and macrobeads did not differ significantly. However, the amount of in vitro PTH secretion from microencapsulated cells was significantly lower than that from macroencapsulated cells, although it increased throughout the incubation period. Immunohistochemistry of PTH staining in both of the encapsulated cells identified as positive after retrieval. Conclusion Contrary to the literature, a minimal in vivo immune response was developed for alginate-encapsulated parathyroid cells, regardless of bead size. Our findings suggest that injectable, micro-sized beads obtained using high-voltage may be a promising method for a non-surgical transplantation approach. [GRAPHICS] . en_US
dc.identifier.citationcount 1
dc.identifier.doi 10.1007/s40618-023-02075-7
dc.identifier.endpage 2267 en_US
dc.identifier.issn 0391-4097
dc.identifier.issn 1720-8386
dc.identifier.issue 11 en_US
dc.identifier.pmid 36976484
dc.identifier.scopus 2-s2.0-85151129323
dc.identifier.scopusquality Q1
dc.identifier.startpage 2257 en_US
dc.identifier.uri https://doi.org/10.1007/s40618-023-02075-7
dc.identifier.uri https://hdl.handle.net/20.500.14517/1280
dc.identifier.volume 46 en_US
dc.identifier.wos WOS:000960412100001
dc.identifier.wosquality Q1
dc.language.iso en
dc.publisher Springer en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.scopus.citedbyCount 2
dc.subject Electric field en_US
dc.subject Microbead en_US
dc.subject Transplantation en_US
dc.subject Hypoparathyroidism en_US
dc.subject Parathyroid cell encapsulation en_US
dc.title Microencapsulation of parathyroid cells via electric field and non-surgical transplantation approach en_US
dc.type Article en_US
dc.wos.citedbyCount 2

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