Mirtazapine suppresses sterile inflammation through NLRP3-inflammasome in diabetic rat kidney

dc.authorid Can, Ozgur/0000-0002-2260-3174
dc.authorid Sahinturk, Varol/0000-0003-2317-3644
dc.authorid Sahin, Erhan/0000-0003-2152-0542
dc.authorscopusid 56022477500
dc.authorscopusid 57008316500
dc.authorscopusid 57008255100
dc.authorscopusid 6603103062
dc.authorscopusid 12804536700
dc.authorscopusid 6507308729
dc.authorwosid Can, Ozgur/ABI-6843-2020
dc.authorwosid Sahinturk, Varol/V-5195-2017
dc.authorwosid Sahin, Erhan/AAA-2830-2021
dc.contributor.author Sahin, Erhan
dc.contributor.author Bektur, Ezgi
dc.contributor.author Donmez, Dilek Burukoglu
dc.contributor.author Baycu, Cengiz
dc.contributor.author Can, Ozgur Devrim
dc.contributor.author Sahinturk, Varol
dc.date.accessioned 2024-05-25T11:25:16Z
dc.date.available 2024-05-25T11:25:16Z
dc.date.issued 2019
dc.department Okan University en_US
dc.department-temp [Sahin, Erhan; Bektur, Ezgi; Donmez, Dilek Burukoglu; Sahinturk, Varol] Eskisehir Osmangazi Univ, Med Sch, Histol & Embryol Dept, Eskisehir, Turkey; [Baycu, Cengiz] Okan Univ, Histol & Embryol Dept, Med Sch, Istanbul, Turkey; [Can, Ozgur Devrim] Anadolu Univ, Pharmacol Dept, Fac Pharm, Eskisehir, Turkey en_US
dc.description Can, Ozgur/0000-0002-2260-3174; Sahinturk, Varol/0000-0003-2317-3644; Sahin, Erhan/0000-0003-2152-0542 en_US
dc.description.abstract Aim The aim of this study was to investigate the effects of mirtazapine, which is anti-oxidative and antidepressant agent, on the kidney damage caused by diabetes mellitus. Materials and methods: The rats were randomly divided into three groups (n = 7 animals in each group). The group I rats served as control and they received 0.1 mol/L of citric acid buffer (pH = 4.5) as vehicle. The rats in the group II (DM group) and III (DM + Mirtazapine-treated group) were treated intraperitoneally with a single dose of 55 mg/kg streptozotocin dissolved in 0.1 mol/L of citric acid buffer. Group DI rats were also received 20 mg/kg/day of mirtazapine for 2 weeks. At the end of the experiment, the rats were sacrificed. Then, the kidneys were excised and prepared for microscopical examination, caspase-1 and NLRP3 proteins were examined using immunohistochemistry and western blotting. The TUNEL assay for apoptosis and ELLSA assay for IL-1 beta were performed. Results: Histological examination showed that mirtazapine administration has an ameliorative effect on DM-induced kidney damage. Immunohistochemical and western blot analyses showed that NLRP3 and caspase-1 expressions were increased in the DM group according to the control group and the mirtazapine administration decreased these expressions. The intraglomerular and tubular TUNEL-positive cells were numerous in the DM group compared to the mirtazapine-treated group. The level of IL-1 beta was highest in the DM group, and decreased significantly in the mirtazapine-treated group. Conclusion: In this study, 20 mg/kg/day mirtazapine administration for 2 weeks reduced NLRP3 and caspase-1 expressions and IL-1 beta level in the diabetic rat kidneys. These results suggesting that mirtazapine may be useful in the treatment of DM and other metabolic diseases. Advanced molecular studies are needed to elucidate the exact effects of mirtazapine on NLRP3 inflammasome. en_US
dc.identifier.citationcount 15
dc.identifier.doi 10.1016/j.acthis.2019.01.007
dc.identifier.endpage 296 en_US
dc.identifier.issn 0065-1281
dc.identifier.issn 1618-0372
dc.identifier.issue 3 en_US
dc.identifier.pmid 30711241
dc.identifier.scopus 2-s2.0-85060766325
dc.identifier.scopusquality Q3
dc.identifier.startpage 289 en_US
dc.identifier.uri https://doi.org/10.1016/j.acthis.2019.01.007
dc.identifier.uri https://hdl.handle.net/20.500.14517/872
dc.identifier.volume 121 en_US
dc.identifier.wos WOS:000466261400005
dc.identifier.wosquality Q4
dc.institutionauthor Baycu C.
dc.language.iso en
dc.publisher Elsevier Gmbh en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.scopus.citedbyCount 16
dc.subject Diabetes mellitus en_US
dc.subject Kidney en_US
dc.subject Mirtazapine en_US
dc.subject NLRP3 en_US
dc.subject Caspase-1 en_US
dc.title Mirtazapine suppresses sterile inflammation through NLRP3-inflammasome in diabetic rat kidney en_US
dc.type Article en_US
dc.wos.citedbyCount 18

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